Multichannel-systems MEA1060-Up-BC Manual Bedienungsanleitung

MEA Amplifier with Blanking Circuit for Upright Microscopes Manual

MEA Amplifier with Blanking Circuit for Upright Microscopes 10 3.2 Installing the Software System requirements Software: One of the following Microsof

First Use of the MEA Amplifier 11 3.3 MEA_Select Program MEA Amplifier with blanking circuit (MEA1060-BC) When starting the software program MEA_Sele

MEA Amplifier with Blanking Circuit for Upright Microscopes 12 A blanking signal (TTL pulse) transiently switch off the input stage of the amplifier d

First Use of the MEA Amplifier 13 The command List Mode opens an additional window: List of Stimulation Patterns. In List Mode you can download a list

MEA Amplifier with Blanking Circuit for Upright Microscopes 14 3.4 Setting Up and Connecting the MEA Amplifier Warning: Spilled liquid can damage or

First Use of the MEA Amplifier 15 Please see also the separate handout "MEA Microelectrode Systems" for setup suggestions with detailed illu

MEA Amplifier with Blanking Circuit for Upright Microscopes 16 Connecting the amplifier to the stimulus generator The following illustration shows the

17 4 First Tests and Tutorial 4.1 First Functional Tests Each MEA amplifier has been thoroughly tested at the factory site before delivery. However

MEA Amplifier with Blanking Circuit for Upright Microscopes 18 The following screen shot shows a recording from a typical MEA amplifier with a test m

First Tests and Tutorial 19 4.3 Computer Connection Testing the computer connection The MEA_Select program automatically detects and lists all serial

Information in this document is subject to change without notice. No part of this document may be reproduced or transmitted without the express wri

MEA Amplifier with Blanking Circuit for Upright Microscopes 20 4.4.1 Operating multiple MEA Amplifiers with one STG It is possible to operate more th

First Tests and Tutorial 21 To start and stop only the channels associated with one amplifier, use the Start / Stop buttons for individual triggers. E

MEA Amplifier with Blanking Circuit for Upright Microscopes 22 1. Under Stimulation, select Ground. 2. Click any electrodes that you like to ground

First Tests and Tutorial 23 Stimulation without blanking Usually, you will always use the blanking when you stimulate. But it is possible to switch of

MEA Amplifier with Blanking Circuit for Upright Microscopes 24 The stimuli are sent to the selected stimulating electrodes. You see stimulus artifacts

First Tests and Tutorial 25 You see that there are no stimulus artifacts visible on almost all non-stimulating electrodes, with some residual artifac

27 5 Operating the MEA Amplifier 5.1 Signal Amplification and Filters The MEA amplifier with blanking circuit is a 60-channel preamplifier with a br

MEA Amplifier with Blanking Circuit for Upright Microscopes 28 For information on the gain and filters of the MC_Card data acquisition system, please

Troubleshooting 29 DC offset correction The internal baseline restore routine of the preamplifier restores the offset to the ground level, that is, to

iii Table of Contents 1 Introduction 5 1.1 About this Manual 5 2 Important Information and Instructions 7 2.1 Operator's Obligations 7

MEA Amplifier with Blanking Circuit for Upright Microscopes 30 5.3 Mounting the MEA Probe and Grounding the Bath Warning: Do not use too much force.

Troubleshooting 31 1. Attach the provided silver wire or pellet to the amplifier's ground and place it into the bath, near the rim of the cultur

MEA Amplifier with Blanking Circuit for Upright Microscopes 32 1. Under Stimulation, select Ground. 2. Click any electrodes that you like to grou

Troubleshooting 33 5.5 Stimulation Recommended stimulus protocols for MEA electrodes MCS recommends to use only biphasic voltage pulses with the neg

MEA Amplifier with Blanking Circuit for Upright Microscopes 34 Important: You can only stimulate during an active TTL pulse, which is used for trigger

Troubleshooting 35 If the List Mode is open, there is an additional button Trace List. If the Trace List button is active all other funct

MEA Amplifier with Blanking Circuit for Upright Microscopes 36 5.6 Stimulus Artifact Suppression (Blanking) A TTL pulse (blanking signal) that has to

Troubleshooting 37 Wait settings A delay (up to 5000 μs) between stopping stimulation (end of TTL pulse) and restarting the recording can be programme

MEA Amplifier with Blanking Circuit for Upright Microscopes 38 Single pulses versus repeated pulses Interestingly, the behavior of MEA electrodes when

Troubleshooting 39 Behavior of stimulation electrodes Due to the high electric charge that is applied by the stimulus and the general slow kinetics of

MEA Amplifier with Blanking Circuit for Upright Microscopes iv 6 Troubleshooting 44 6.1 About Troubleshooting 44 6.2 Error Messages 44 6.3 No

MEA Amplifier with Blanking Circuit for Upright Microscopes 40 Stimulus artifacts with switched off blanking on a typical non-stimulating 30 μm electr

Troubleshooting 41 60MEA200/10iR-Ti Voltage driven stimulation (1 V, electrode 33) on 10 μm TiN electrodes. The stimulus artifact suppression is slig

MEA Amplifier with Blanking Circuit for Upright Microscopes 42 Voltage driven stimulation (1 V, electrode 33) on 100 μm gold electrodes. You see only

Troubleshooting 43 The response of the cells to the stimulation is instantaneous in the cells near to the pulse. The cells recorded in the periphery

MEA Amplifier with Blanking Circuit for Upright Microscopes 44 6 Troubleshooting 6.1 About Troubleshooting The following hints are provided to solve s

Troubleshooting 45 MEA electrode is defective See also the MEA manual. Possible causes: ? The contact pads are contaminated.  Clean the contact pad

MEA Amplifier with Blanking Circuit for Upright Microscopes 46 6.5 Artifacts on All Channels You see strange artifacts on all channels. This behavior

Troubleshooting 47 6.6 Unpredictable Noise and Artifacts You use the amplifier for stimulation and you see unpredictable noise and / or artifacts on

MEA Amplifier with Blanking Circuit for Upright Microscopes 48 Due to the higher electrode impedance, the maximum input voltage of the MEA1060-BC amp

Troubleshooting 49 6.8 Technical Support Please read the Troubleshooting part of the manual or help first. Most problems are caused by minor handling

5 1 Introduction 1.1 About this Manual This manual comprises all important information about the first installation of the hardware and software, a

51 7 Appendix 7.1 Technical Specifications 7.1.1 Pin and MEA Layout The layout of standard MEA electrodes follows the scheme of a standard grid: The

MEA Amplifier with Blanking Circuit for Upright Microscopes 52 Assignment pin to electrode 68-pin socket MEA electrode 68-pin socket MEA

Appendix 53 7.1.2 MEA1060-Up-BC Operating temperature 10 °C to 35 °C Storage temperature 0 °C to 50 °C Dimensions (W x D x H) 165 mm x 165 mm x

MEA Amplifier with Blanking Circuit for Upright Microscopes 54 7.1.3 FA60S-BC Operating temperature 10 °C to 50 °C Storage temperature 0 °C to 5

Appendix 55 7.2 Contact Information Local retailer Please see the list of official MCS distributors on the MCS web site. User forum The Multi Channel

MEA Amplifier with Blanking Circuit for Upright Microscopes 56 USB-MEA-Systems Product Product Number Description USB-MEA-System for upright microsco

Appendix 57 USB-MEA-System with blanking circuit for inverted microscopes with advanced perfusion USB-MEA240-Inv-4-BC-System-E electrodes, 4 analog c

MEA Amplifier with Blanking Circuit for Upright Microscopes 58 Accessories Product Product Number Description Adapter for MEAs with 8 special electr

Appendix 59 Spare Parts Product Product Number Description Heating element with 8 mm bore hole HE-Inv-8 Heating element with 12 mm bore hole HE-Inv-1

MEA Amplifier with Blanking Circuit for Upright Microscopes 60 MEA-Systems Product Product Number Description MEA recording system for inverted micr

61 8 Index

MEA Amplifier with Blanking Circuit for Upright Microscopes 62

63 3 3D-MEA 37 A Aliasing 25 B Band pass band...25 Bandwidth 25 C Change MEA 28 COM serial port ...

64

7 2 Important Information and Instructions 2.1 Operator's Obligations The operator is obliged to allow only persons to work on the device, who

MEA Amplifier with Blanking Circuit for Upright Microscopes 8 2.3 Important Safety Advice Warning: Make sure to read the following advice prior to i

First Use of the MEA Amplifier 9 3 First Use of the MEA Amplifier 3.1 Welcome to the MEA Amplifier Raw data from up to 60 electrodes of a microelec