Multichannel-systems MC_Rack Manual Bedienungsanleitung

MC_Rack Manual

MC_Rack Manual 4 The MC_Rack help is divided into the following main sections:  Step by Step Tutorial: The tutorial introduces basic MC_Rack feature

MC_Rack Manual 94 4.3.4 Averaged Data Averaged Data, that is, a data stream generated by an Averager, is a special kind of triggered data. Only the la

MC_Rack Features 95 Click the Window tabbed page and select the option Start on Trigger. Select the Avg Trigger that was recorded together with the Av

MC_Rack Manual 96 4.3.5 Digital Data and Binary Code Only two voltage levels are allowed as an input of the 16 digital channels: 0 Volts or 5 Volts.

MC_Rack Features 97 4.4 Recorder 4.4.1 Recording Data In MC_Rack, the rack you use to record and analyze data online and offline, and the data files

MC_Rack Manual 98 1. Click the data stream that you are interested in, that is generally the Electrode Raw Data stream. The available electrode chann

MC_Rack Features 99 Recording triggered data When you record data that is triggered by an event, for example the response to a stimulation, please mak

MC_Rack Manual 100 It is important to know that MC_Rack never modifies or overwrites any existing data file. That means, in an offline analysis (using

MC_Rack Features 101 4.5 Replayer 4.5.1 Loading a Data File Data files can be stored separate from the rack files. Use the Replayer to load a data fi

MC_Rack Manual 102 4.5.2 File Specifications Some information about the experiment is saved together with the data. This information is displayed on

MC_Rack Features 103 Hint: If you want to graph (already extracted) parameters, such as the spike rate, without a further offline analysis, replay on

First Use of MC_Rack 5 Recommended operating system settings The following automatic services of the Windows operating system interfere with the data

MC_Rack Manual 104 4.6 Displaying Data 4.6.1 Display Types You can flexibly define the electrode grid for all displays. You can define not only the ch

MC_Rack Features 105 The channels of MEA A are tagged with an "A". Likewise, the channels of MEA B are tagged with a "B". If you

MC_Rack Manual 106 4.6.3 Display Settings You can select the range and refresh rate of the display from the drop-down lists. In addition, you can zo

MC_Rack Features 107  Zoom into a part of the displayed sweep by clicking and dragging the sliders with the mouse.  Or select a slider with the mo

MC_Rack Manual 108 With Peak Detection, the highest (max) and the lowest (min) value of the five data points are taken and connected by straight verti

MC_Rack Features 109 4.6.5 Customizing a Display Showing and hiding the axes bar * You can hide the axes bar on top of the display to save space and o

MC_Rack Manual 110 7. Type in the number of the electrode according to the MEA layout, for example 78. The data traces appear on top of the picture.

MC_Rack Features 111 4.7 Data Display (for Monitoring Raw Data) 4.7.1 Plot Types Click to add a Data Display to your rack. You can choose between d

MC_Rack Manual 112 ASCII Export The created file has the following structure.  The first column is the x-axis (time).  Each following column repre

MC_Rack Features 113 Using a raster plot The Raster plot is only available for spike data streams. Make sure you have a Spike Sorter in the rack, or

MC_Rack Manual 114 4.8 Digital Display (for Monitoring TTL Inputs) 4.8.1 Displaying Digital Data The Digital Display is used to view either the logic

MC_Rack Features 115 See "About Digital Data and Binary Code" for MC_Rack Features. 1. On your rack, click the Digital Display. 2. Click th

MC_Rack Manual 116 Click Trigger tab. Select “Manual Trigger” from the Channel drop down menu. When MC_Rack is displaying or recording data, the butt

MC_Rack Features 117 To display data and manual trigger, please select the desired data streams in the “Data” tab of the display. The display has to

MC_Rack Manual 118 You can select the digital input channels that will be used by the Trigger Detector. All other channels are masked, that is, are n

MC_Rack Features 119 4.9.4 Triggering on Biological Signals You can define a threshold for triggering on biological (analog) signals from Analog Raw

MC_Rack Manual 120 4.9.5 Triggering on Extracted Parameters Similar to the triggering on raw data streams, you can also define a threshold for an ext

MC_Rack Features 121 Single or periodic trigger event If you use a periodic trigger, you define the start time (Wait), when the trigger occurs the fi

MC_Rack Manual 122 4.10 Automatic Feedback 4.10.1 Digital Output To use this feature, you need a DigOut connector for your computer and the MC_Card dr

MC_Rack Features 123 4.11 Real-time Feedback 4.11.1 Real-time Feedback Introduction The real-time feedback feature represents a significant improvemen

7 3 Step by Step Tutorial 3.1 Using the Step by Step Tutorial In the following tutorial, you will set up virtual racks for different applications f

MC_Rack Manual 124 Setting up the Hardware To generate real-time feedback stimulation to a biological sample, you need an advanced data acquisition sy

MC_Rack Features 125 The data trace (blue), the detection level (black), and the generated TTL pulses (red) are displayed in one window. It is possibl

MC_Rack Manual 126 When recording with a MEA2100-System, the digital display is labeled: The first four rows display the “DigIn” channels for bit 0 to

MC_Rack Features 127 Digital Hardware Filter In the "Digital Hardware Filter" tab, it is possible to switch the real-time feedback feature o

MC_Rack Manual 128 Note: Please use first order filters whenever possible. Higher order filters tend to be unstable and oscillate, especially when us

MC_Rack Features 129 A time interval of 500 ms is used to calculate the standard deviation. You can set the factor by which the standard deviation is

MC_Rack Manual 130 Select the “Event Duration” in milliseconds from the numeric updown box. The event duration is the time after a crossing of the de

MC_Rack Features 131 Select from the “Spike Count” drop down menu either “Single Spike” or values from 2 to 100. This corresponds with the number of s

MC_Rack Manual 132 In case of rate detection, the logical state becomes TRUE from the detection point of the last event that is needed to fulfill the

MC_Rack Features 133 Examples: The following examples further illustrate the possible combinations of the Feedback Logic. In example one, single spik

MC_Rack Manual 8 3.2 Monitoring and Recording Activity 3.2.1 Starting MC_Rack  Double-click the MC_Rack icon or select MC_Rack from the Start menu.

MC_Rack Manual 134 4.12 Filtering Data 4.12.1 Filtering Data The Digital Filter tool can be used online or offline. Displays in dependence of filters

MC_Rack Features 135 Adding a filter to your rack 1. To add a virtual filter to your rack, click "Add Filter" from Edit menu, or click the

MC_Rack Manual 136 Signal distortion Though filters present an easy and convenient way to remove unwanted signals from your data, filters should be us

MC_Rack Features 137 4.12.2 Filter Characteristics Bessel A Bessel filter is a type of linear filter with a maximally flat group delay (maximally line

MC_Rack Manual 138 Bandstop Resonator In signal processing, a bandstop filter is a filter that passes most frequencies unaltered, but attenuates thos

MC_Rack Features 139 Example: This can be demonstrated by the following experiment. A regular 1000 Hz signal (from a pulse generator) is applied to el

MC_Rack Manual 140 4.12.4 Downsampling The MC_Rack downsampling feature offers the possibility to generate raw and filtered data streams with differen

MC_Rack Features 141 MC_Rack sets the cutoff frequency of the filter automatically. A second dialog will appear and prompt the user to select a cutof

MC_Rack Manual 142 4.13 Spike Sorter 4.13.1 Spike Detection Methods The virtual Spike Sorter instrument is used for extracting spikes from the raw dat

MC_Rack Features 143 4.13.2 Detecting Spikes by a Threshold You can define a threshold (spike detection level) for each channel separately. All peaks

Step by Step Tutorial 9 In the Recorder settings, you select the data streams and channels that you want to save to the hard disk. You define the path

MC_Rack Manual 144 Only waveforms that satisfy both criteria (slope and amplitude) are considered spikes. See also the preceding illustrations "

MC_Rack Features 145 4.13.5 Spike Sorting Most often, spikes do not represent a single unit activity, but are derived from multiple neurons. Spikes fr

MC_Rack Manual 146 The following illustration shows the activity of two neurons, as spike cutouts on a small time basis. The first (red) signal has on

MC_Rack Features 147 4.13.6 Burst Analysis The “Burst Detection” option allows the detection and analysis of spike bursts. The spikes have to be detec

MC_Rack Manual 148 The detection parameter for bursts are shown in the following picture. Parameter for burst detection: Max. interval to start burst

MC_Rack Features 149 Different units on each electrode, separated by the spike sorter, will be shown in different colors. In the same window, a color

MC_Rack Manual 150 1. Channel / Unit 2. Number of Spikes 3. Mean Spike Frequency [Hz] 4. Number of Bursts 5. Bursts per Second 6. Bursts pe

MC_Rack Features 151 Add a display to your virtual rack. Click “Data” tab. In “Data Streams” select “Spike parameter 1” and “Timestamp”. In “Plot Type

MC_Rack Manual 152 4.14 Analyzing Data 4.14.1 Analyzing Data Two virtual instruments are provided for data analysis: Standard Analyzer based on discre

MC_Rack Features 153 4.14.2 Time-Interval Based Analyzer Extracted Parameters The following parameters can be extracted with the Analyzer from the des

MC_Rack Manual 10 MEA60-System Select 2 dimensional (MEA) if you are performing extracellular recordings from microelectrode arrays (MEA) with a MEA60

MC_Rack Manual 154 Slope extraction The 10 % / 90 % (or 20 / 80 or 30 / 70) interval of the peak-peak amplitude (stretching from minimum to maximum)

MC_Rack Features 155 1. Set up a rack with a Trigger Detector and an Analyzer. 2. Click the ROI tab of the Analyzer. 3. Select the option Start on T

MC_Rack Manual 156 Maximum trigger rate Please note that the ROI must be defined in a way that the trigger rate does not cause ROIs to overlap. The m

MC_Rack Features 157 When monitoring faster signals like neuronal spikes, a bin size of 1 s will generally be fine. For special applications, it can

MC_Rack Manual 158 4.14.3 Event-Based Spike Analyzer Extracted Parameters (Spike Analyzer) The following parameters can be extracted with the Spike An

MC_Rack Features 159 4.15.3 False Color Maps In the Color vs. Time and the False Color plot, color values or gray-scale intensities are assigned to pa

MC_Rack Manual 160 You can choose between four different false color maps. The False Color plot shows only the current values as colors without inform

MC_Rack Features 161 4.15.7 Parameter/Digital Display Tools + Settings Each Parameter Display or Digital Display has a toolbar at the top of the windo

MC_Rack Manual 162 Note: In general, all buttons apply only to the displayed channels. If, for example, 64 channels are displayed, moving and zooming

MC_Rack Features 163  Move the mouse pointer over the clamp control cursor / measure tool until the mouse pointer becomes a hand and then press the

Step by Step Tutorial 11 MEA120-System Select 2 dimensional ( MEA) if you are performing extracellular recordings from microelectrode arrays (MEA) wit

MC_Rack Manual 164 4.15.8 ASCII Export of Extracted Parameters Click to save the complete data streams of the displayed channels as a tab-delimited

MC_Rack Features 165 4.16 Averager 4.16.1 Averaging Data Sweeps The Averager adds up triggered sweeps successively. You can use the Averager to reduce

MC_Rack Manual 166 Note: When recording averaged data, you can save the data only continuously. The averaged data stream is recorded "triggered&

MC_Rack Features 167 4.17.2 Audio Out with USB-ME Data Acquisition Using an USB-ME device you can additionally transfer electrode activity into sound

169 5 Data Export 5.1 About Data Export The data acquisition and analysis program from Multi Channel Systems MC_Rack provides tools for a user frien

MC_Rack Manual 170 Spike2 CED .mcd (MC_Rack) Spikes, field potentials Analysis, for example spike sorting, PSTH, correlational analyses, filters, te

Data Export 171 5.2 Graphs for Presentations 5.2.1 Low Resolution Pics (Screen Shots)  You can use the Image Capture feature of the Parameter Displ

MC_Rack Manual 172 You can also use the commercially available program DataAccess Pro from Bruxton (www.bruxton.com) to open * .mcd files directly and

Data Export 173 Spike2 automatically creates templates from raw spike data based on user-defined parameters. Spike2 can cluster spike data using princ

MC_Rack Manual 12 ME-Systems Select 1 dimensional if you are using a ME-System (generally used for in vivo or special in vitro applications). ME-Syste

MC_Rack Manual 174 5.4.2 Waveform Shape and QT Interval For drug screenings, the analysis of the waveform, especially of the field potential duration

175 6 Troubleshooting 6.1 Technical Support Please read the Troubleshooting part of the MC_Rack Manual / Help first. Most problems are caused by mino

MC_Rack Manual 176 6.2 Error Messages 6.2.1 Computer Performance Performance limit messages occur if the computer performance does not support the cu

Troubleshooting 177 6.2.2 Replayer Occurs when a rack file is opened and the file path of the data file loaded into the Replayer is not valid. The O

MC_Rack Manual 178 6.2.3 Recorder Occurs when the "Window Extent" setting in "Windows" tab of the Recorder is out of range. Pleas

Troubleshooting 179 6.3 Data is not Written to Hard Disk You started the recording by pressing the Start button. You see the recorded data in the MC_

MC_Rack Manual 180 Signals on the analog channels are recorded "as is", with no respect to the gain specified in MC_Rack, as the true gain i

Troubleshooting 181 7 Appendix 7.1 Contact Information Local retailer Please see the list of official MCS distributors on the MCS web site. User forum

183 8 Glossary A aliasing: Frequencies (noise) that are above half the sampling rate (for example above 2.5 kHz at a 5 kHz sampling rate) will be tr

Step by Step Tutorial 13 Data Source Configuration Click "Configuration". Configuration is an option that can also be used with MC_Card dat

MC_Rack Manual 184 E Enhanced Meta File (EMF): Intermediate format for exchanging vector graphics data, file extension *.emf EPSP: Excitatory postsyna

Glossary 185 O OTC: Organotypic Culture: An MEA experiment where the slice is cultured on the MEA for weeks or months, in contrast to an acute slice e

187 9 Index

MC_Rack Manual 188

189 1 1-dimensional data source setup...73 2 2-dimensional data source setup...73 A Add Digital

MC_Rack Manual 190 Longterm Display...36 plot types...118 settings ...

Index 191 for presentations...190 high resolution...191 Grouping Multitrode ...

MC_Rack Manual 192 O Offset defining a channel offset in channel maps for non-rectangular grids...111 Offset Correction 88 On Trigger 105 Oscillos

Index 193 File Source ...93 Sine Waves Simulation mode...93 Slope analyzing slope ..

Information in this document is subject to change without notice. No part of this document may be reproduced or transmitted without the express wri

MC_Rack Manual 14 In MEA120-System it is possible to configure both amplifiers independent of each other. Additionally it is possible to configure the

MC_Rack Manual 194 W8-System 33, 73 Wait automatic trigger...129 White display background color ...112 Window spi

195

Step by Step Tutorial 15 USB-ME16-FAI-System The USB-ME16-FAI System does not require a MC_Card, but uses an internal data acquisition. Data can be t

MC_Rack Manual 16 USB-ME32-FAI-System The USB-ME32-FAI System does not require a MC_Card, but uses an internal data acquisition. Data can be transferr

Step by Step Tutorial 17 USB-ME64 / USB-ME128 / USB-ME256 Data Acquisition The USB-ME64 / 128 / 256 data acquisition systems are in principle the same

MC_Rack Manual 18 Click Configuration in Source Layout. Choose 256 as total number of channels from the drop down list under No. of Channels. In USB-

Step by Step Tutorial 19 On the Edit menu, click Advanced Configuration to configure the software according to the USB-ME256 hardware. Please see Adv

MC_Rack Manual 20 USB-MEA256 Data Acquisition and Filter Amplification The USB-MEA256 is an external data acquisition device with integrated filter am

Step by Step Tutorial 21 Wireless Recording System The wireless in vivo recording system is the all-in one solution for amplifying, recording, and an

MC_Rack Manual 22 MEA2100-System The MEA2100 recording system is an all-in one solution consisting of headstage and interface board. The MEA2100-Syste

Step by Step Tutorial 23 In MEA2100-System it is possible to configure two headstages independent of each other. MC_Rack identifies two connected head

iii Table of Contents Introduction 1 About this Manual 1 Terms of Use 2 Limitation of Liability 2 Important Safety Advice 2 First Use of MC_Rack

MC_Rack Manual 24 3.2.3 Advanced Configuration Important: This feature can only be used when working with the USB based data acquisition systems USB-M

Step by Step Tutorial 25 Operating 256 channels with USB-ME256 device with one instance of MC_Rack Using an USB-ME256 device, it is possible to operat

MC_Rack Manual 26 Operating 256 channels with USB-ME256 device with four instances of MC_Rack Using an USB-ME256 or an USB-ME128 device, it is possibl

Step by Step Tutorial 27 7. Open the third instance of MC_Rack software program. 8. Click Edit: Data Source Setup and select Block 3 (Channel 129...

MC_Rack Manual 28 Opening an existing Rack File with USB-ME256 and multiple Instances of MC_Rack If you build up (and saved) a complicated rack in the

Step by Step Tutorial 29 Operating two MEA2100-32-Systems with multiple instances of MC_Rack Using a MEA2100-32-Systems with two MEAs or two headstage

MC_Rack Manual 30 5. Start the second instance of MC_Rack. 6. Click "Data Source Setup" in Edit menu. Select the headstage you want to co

Step by Step Tutorial 31 Availability of Additional Analog Channels in USB-ME256 (and USB-ME128 respectively) Depending on the selected "Source

MC_Rack Manual 32 In the "Layout" tab, select the "Default Map". The additional analog channels A1 to A4 are displayed. It is als

Step by Step Tutorial 33 1. Click the electrode array symbol on the toolbar or click Add MC_Card on the Edit menu. The program detects the MC_Card

MC_Rack Manual iv Recorder 97 Recording Data 97 Generating Data Files 99 Replayer 101 Loading a Data File 101 File Specifications 102 Replaying

MC_Rack Manual 34 3. Click the Hardware tab. Here, you can define hardware related parameters. Please note that the amplifier gain is an intrinsic pro

Step by Step Tutorial 35 3.2.5 Channel Tool The Channel Tool feature in MC_Rack allows the selection of one MEA electrode as reference electrode. The

MC_Rack Manual 36 3.2.6 Monitoring Activity Continuously Next, you need a Data Display for monitoring the ongoing activity continuously. Click on the

Step by Step Tutorial 37 Defining the display layout Note: You can set up any channel layout that meets your requirements and save it for later use. Y

MC_Rack Manual 38 4. Click any electrode number that you want to change and select the desired channel number from the Channel list, or type the chann

Step by Step Tutorial 39 Additional windows appear: Channel Offset, X- and Y- Offset and X- and Y-Extend. These commands concern the data display lay

MC_Rack Manual 40 Customized Display 1. Click User Defined Map. 2. Set up a MEA layout with a 5 x 5 grid using Rows and Columns. The MEA layout app

Step by Step Tutorial 41 Selecting data streams 1. Click the Data tabbed page. In this page, you select the data streams that you want to monitor in

MC_Rack Manual 42 3.2.7 Recording Data MC_Rack's philosophy is to strictly separate the actions of all virtual instruments in a rack. That means,

Step by Step Tutorial 43 Choosing the file name and path 1. Click the Recorder tab. 2. Browse your folders and select a path. 3. Type a file name i

Table of Contents v Parameter + Spike Analyzer Display 158 Plot Types 158 Trace Plot 158 False Color Maps 159 False Color vs. Time (Plot) 159 F

MC_Rack Manual 44 Continuous recording versus triggered recording For recording evoked activity, like LTP experiments or retina recordings, it does ge

Step by Step Tutorial 45 3.3 Monitoring and Recording Triggered Activity 3.3.1 Triggering MC_Rack on the Stimulus When recording evoked responses, s

MC_Rack Manual 46 From the Channel list, select the Digital Data D1 channel. A button array appears, one button for each digital input bit. With th

Step by Step Tutorial 47 3.3.2 Monitoring Triggered Activity Next, you need a Data Display for monitoring the ongoing activity. Click on the toolbar

MC_Rack Manual 48 Adjusting the ranges 1. Switch to the Display 1 window. 2. You can zoom in the signals by choosing the appropriate range of the y-a

Step by Step Tutorial 49 1. You can now either select all channels by clicking the check box next to the Electrode Raw Data stream name, or you can p

MC_Rack Manual 50 Triggering the recording Again, the displayed data is independent from the recorded data. Thus we need to start the recording on th

Step by Step Tutorial 51 3.4 MEA2100-System 3.4.1 MEA2100-System Specific Functions of the MEA2100-System The following chapter summarizes all functio

MC_Rack Manual 52 After opening MC_Rack as many times as necessary, please open the “Data Source Setup” dialog from the “Edit” menu. This dialog can o

Step by Step Tutorial 53 The appropriate “Amplifier Gain” factor of the MEA2100-System will automatically be entered. The “Input Voltage Range of Data

MC_Rack Manual 54 The following dialog appears. Select the tab of any of the three stimulation channels (Stimulus 1, 2 or 3). In the "Stimulus&q

Step by Step Tutorial 55 Warning: Anodic (positive) pulses can lead to a formation of titanium oxide on the MEA electrodes. When using MEA electrode

MC_Rack Manual 56 Starting and stopping the stimulation In the "Trigger" drop down menu it is possible to choose different types of triggers

Step by Step Tutorial 57 Selection of stimulation electrodes Click the “Channels” tab to assign the programmed stimulation patterns to individual elec

MC_Rack Manual 58 Dedicated Stimulation Electrodes The function “Dedicated Stimulation Electrodes” can be used to further avoid stimulation artifacts

Step by Step Tutorial 59 When the List Mode is activated, the three stimulator units are coupled to a single starting trigger. The starting trigger is

MC_Rack Manual 60 The command “Remove” eliminates the pattern in the list, which is highlighted in blue. With “Up” or “Down” you can move the pattern

Step by Step Tutorial 61 To monitor the digital data stream you can use the “Digital Display” tool. If a MEA2100 device is connected, the input bits w

MC_Rack Manual 62 Triggering on Stimulation Pulses from Internal STGs It is possible to trigger on stimulation pulses from any of the three internal s

Step by Step Tutorial 63 Setting up the Real-time Feedback Software The "Real-time Feedback" works the same way as with the other data acqui

1 1 Introduction 1.1 About this Manual This manual comprises all important information about MC_Rack. It is assumed that you have already a basic un

MC_Rack Manual 64 3.5 Wireless-System 3.5.1 Wireless System When using a wireless in vivo recording system, the “Hardware” tab is modified. Please se

Step by Step Tutorial 65 The button “Switch Off” can be used to send the currently selected headstage from “Stand By” mode to “Switched Off” mode, for

67 4 MC_Rack Features 4.1 About MC_Rack Features This section provides more detailed information on all features of MC_Rack. The topics are in a sys

MC_Rack Manual 68 If you use MC_Rack together with devices which do not require a MC_Card, but use an internal data acquisition and USB data transfer,

MC_Rack Features 69 MEA60-System Select 2 dimensional (MEA) if you are using a microelectrode array (MEA) and a MEA60-System with 60 electrode channel

MC_Rack Manual 70 MEA120-System Select 2 dimensional (MEA) if you are using two MEA1060 amplifiers and a MEA120-System with 120 electrode channels, 7

MC_Rack Features 71 Linear (custom) Layouts / ME-Systems Select 1 dimensional if you are using any other type of data source, for example, a ME-System

MC_Rack Manual 72 Data Source Configuration Click "Configuration". Configuration is an option that can also be used with MC_Card data acqui

MC_Rack Features 73 In MEA120-System it is possible to configure both amplifiers independent of each other. Additionally it is possible to configure t

MC_Rack Manual 2 1.2 Terms of Use You are free to use MC_Rack for its intended purpose. You agree that you will not decompile, reverse engineer, or o

MC_Rack Manual 74 USB-ME16-FAI-System The USB-ME16-FAI-System does not require a MC_Card, but uses an internal data acquisition. Data can be transfer

MC_Rack Features 75 USB-ME32-FAI-System The USB-ME32-FAI-System does not require a MC_Card, but uses an internal data acquisition. Data can be transfe

MC_Rack Manual 76 USB-ME64 / USB-ME128 / USB-ME256 Data Acquisition The USB-ME64 / 128 / 256 data acquisition systems are in principle the same device

MC_Rack Features 77 Click Configuration in Source Layout. Choose 256 as total number of channels from the drop down list under "No. of Channels&q

MC_Rack Manual 78 On the Edit menu, click Advanced Configuration to configure the software according to the USB-ME256 hardware. Please see Advanced C

MC_Rack Features 79 USB-MEA256 Data Acquisition and Filter Amplification The USB-MEA256 is an external data acquisition device with integrated filter

MC_Rack Manual 80 Wireless Recording System The wireless in vivo recording system is the all-in one solution for amplifying, recording, and analyzing

MC_Rack Features 81 MEA2100-System The MEA2100 recording system is an all-in one solution consisting of headstage and interface board. The MEA2100-Sys

MC_Rack Manual 82 In MEA2100-System it is possible to configure two headstages independent of each other. MC_Rack identifies two connected headstages

MC_Rack Features 83 4.2.2 Defining Hardware Settings Before you start building a rack, configure first the software according to your hardware and am

3 2 First Use of MC_Rack 2.1 Welcome to MC_Rack Please read the following paragraphs to understand the general idea behind the MC_Rack program befor

MC_Rack Manual 84 Amplifier gain Specify the gain of the amplifier used. The gain is an intrinsic option of the device and can not be altered. Make su

MC_Rack Features 85 Sampling frequency As a rule of thumb, the sampling rate should equal five times the highest signal frequency for a good digitize

MC_Rack Manual 86 4.2.3 Channel Tool The Channel Tool feature in MC_Rack allows the selection of one MEA electrode as reference electrode. The tool wo

MC_Rack Features 87 4.2.4 Grouping Multitrode Channels Channels from electrodes with multiple channels (tetrodes or stereotrodes, for example) can be

MC_Rack Manual 88 1. From the Edit menu, select Data Source Setup. Data Source Setup is only available as long as your current virtual rack is empty (

MC_Rack Features 89 Open a binary file in MC_Rack in simulation mode. Data of this file is repeated in a infinite loop.

MC_Rack Manual 90 4.3 Data Streams and Channels 4.3.1 Data Stream Types A data stream can include several channels of the same data type. The term c

MC_Rack Features 91 chapter About Digital Data and Binary Code under MC_Rack Features, General Aspects. Trigger: Generated by a virtual Trigger Detec

MC_Rack Manual 92 If you want to select or deselect all channels of a stream, check the box next to the stream name in the tree view. Note: Click a

MC_Rack Features 93 When using the Replayer for replaying triggered data, make sure all other virtual instruments in your rack are set up according t